Super Resolution Microscope N-SIM
XY resolution | Approx. 100nm (up to 85nm: theoretical, in TIRF-SIM mode, 488nm excitation) |
---|---|
Z-axis resolution | Approx. 300nm |
Image acquisition time | Up to 0.6 sec./frame (TIRF-SIM/2D-SIM) Up to 1 sec. (3D-SIM) (needs more 1-2 sec. for calculation) |
Imaging mode | TIRF-SIM (TIRF XY super resolution) 2D-SIM (XY super resolution, up to 3µm deep) 3D-SIM (XYZ super resolution, up to 20µm deep) |
Multi-color imaging | Up to 5 colors |
Compatible laser | Standard: 488nm, 561nm Option: 457nm, 515nm, 532nm |
Compatible microscopes | Motorized inverted microscope ECLIPSE Ti-E
|
Objectives | CFI Apo TIRF 100x oil (NA 1.49), CFI Plan Apo IR 60x WI (NA 1.27) |
Camera | Andor Technology iXon DU897 EMCCD camera |
Software | NIS-Elements Ar/NIS-Elements C (with confocal microscope A1) |
Operation conditions | 25 °C (± 0.5 °C) |