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  7. N-SIM - Specifications

Super Resolution Microscope N-SIM

XY resolution Approx. 100nm
(up to 85nm: theoretical, in TIRF-SIM mode, 488nm excitation)
Z-axis resolution Approx. 300nm
Image acquisition time Up to 0.6 sec./frame (TIRF-SIM/2D-SIM)
Up to 1 sec. (3D-SIM)
(needs more 1-2 sec. for calculation)
Imaging mode TIRF-SIM (TIRF XY super resolution)
2D-SIM (XY super resolution, up to 3µm deep)
3D-SIM (XYZ super resolution, up to 20µm deep)
Multi-color imaging Up to 5 colors
Compatible laser Standard: 488nm, 561nm
Option: 457nm, 515nm, 532nm
Compatible microscopes Motorized inverted microscope ECLIPSE Ti-E
  • Perfect Focus System
  • Motorized XY stage with encoders
  • Piezo Z stage
Objectives CFI Apo TIRF 100x oil (NA 1.49), CFI Plan Apo IR 60x WI (NA 1.27)
Camera Andor Technology iXon DU897 EMCCD camera
Software NIS-Elements Ar/NIS-Elements C (with confocal microscope A1)
Operation conditions 25 °C (± 0.5 °C)