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  8. C2+ - Specifications

Confocal Microscope C2+

Compatible laser*1 405 nm/440 nm, 488 nm/Ar laser (457 nm, 477 nm, 488 nm, 514 nm), 543 nm/561 nm/594 nm, 633 nm/638 nm/640 nm
Laser unit C-LU3EX 3-laser Module EX (AOM or manual modulation), LU4A 4-laser Module (AOTF modulation)
Standard detector Wavelength: 400-750 nm, Detector: 3PMT, Filter cube: 2 filter cubes
Diascopic detector (option) Wavelength: 400-700 nm, Detector: 1 PMT
Scanning head (galvano) With Standard detector:
Pixel size: 2048 x 2048
Scanning speed: Standard mode: 2 fps (512 x 512 pixels, bi-direction), 17 fps (512 x 32 pixels, bi-direction), Zoom: 1-1000x
Fast mode: 8 fps (512 x 512 pixels, bi-direction), 100 fps (512 x 32 pixels, bi-direction)*2, Zoom: 8-1000x
With spectral detector:
Pixel size: max. 1024 x 1024 pixels
Scanning speed: 0.5 fps (512 x 512 pixels, single direction), max. 6 fps (64 x 64 pixels, single direction)
Scanning mode X-Y, XY rotation, zoom, ROI, XYZ, time lapse, X-Z, stimulation, multipoint, image stitching (large image)
Pinhole Circular shape, 6 size
Spectral detector (with galvano scanner) (option) Number of channels: 32 channels, Wavelength: 400-750 nm, Wavelength resolution: 2.5 nm, 5 nm, 10 nm, wavelength range variable in 0.25 nm steps, Unmixing: High-speed unmixing, precision unmixing
FOV Square inscribed in a ø18 mm circle
Image bit depth 12 bits
Compatible microscopes ECLIPSE Ti-E/Ti-U inverted microscope, ECLIPSE Ni-E (focusing nosepiece type/stage focusing type)/Ni-U upright microscope, ECLIPSE FN1 fixed stage microscope, AZ100 multi-purpose zoom microscope
Z step Ti-E: 0.025 µm, FN1 stepping motor: 0.05 µm, Ni-E: 0.025 µm
NIS-Elements C software Display/image processing/analysis
2D/3D/4D analysis, time-lapse analysis, 3D volume rendering/orthogonal, spatial filters, image stitching, multipoint time-lapse, spectral unmixing, real-time unmixing, virtual filters, deconvolution, AVI image file output
Application: FRAP, FLIP, FRET, photoactivation, colocalization, three-dimensional time-lapse imaging, multipoint time-lapse imaging
Control Computer OS: Microsoft Windows 7 Professional 64 bit SP1 (Japanese/English), CPU: Intel Xeon X3565 (3.20 GHz/8 MB/1066 MHz/Quad Core) or higher, Memory: 4 GB, Hard disk: 146 GB SATA 3 Gb/s (7200 rpm), Data transfer: LAN, Network interface: Gigabit Ethernet, Monitor: 1600 x 1200 or higher resolution, dual monitor, Configuration is recommended
  • *1Compatible lasers and usable wavelengths differ depending on the laser unit in use.
  • *2The described frame rate is NOT available with Rotation, CROP, ROI, Spectral imaging and stimulation.