Inverted Research Microscope ECLIPSE Ti

Scientists have overcome many live cell imaging challenges using advanced techniques such as TIRF, confocal, FRET, photo activation and microinjection. At the center of all this is the Eclipse Ti, a powerful system that provides instant access to all these methods plus revolutionary Nikon CFI60 optics. Available in three models, the Ti series offers improved system speeds, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging.

The diagram shows the case when an immersion type objective is used. A dry type objective is also available.

By now employing 870nm wavelength for the coverglass interface detection, near-infrared fluorescence dyes including Cy5.5 can be used. Nikon offers two PFS models, one for UV-visible wavelength imaging and one for multiphoton imaging. The multiphoton model can correct for focus drift even when imaging with wavelengths ranging from 880-1300 nm.

Multipoint snapshots of HeLa cells transiently expressing Venus-tubulin and mCherry-actin and stained with Hoechst33342 and DiD. (All in pseudo-color)
Photos courtesy of: Kenta Saito and Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University

The operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments. Faster device movement and image acquisition reduce overall light exposure and subsequent photo-toxicity, leading to more meaningful data. The digital Controller Hub significantly increases motorized accessory speed by reducing communication overhead time between components, boosting total operation speed.

The revolutionary external phase contrast unit incorporates a phase ring and allows the use of high NA objective lenses without a phase ring for phase contrast observation. Because there is no light loss due to a phase ring, bright "full intensity" fluorescence images as well as high-resolution phase contrast images can be captured using the same objective lens.

The motorized laser TIRF illuminator was developed for use with Ti-E and Ti-U. The laser incident angle can be easily stored and reproduced with a single touch of the control pad button. This enables alternate time-lapse recording between multi-wavelength TIRF and epi-fluorescence images.

The Ti-E and Ti-U feature a specialized photo activation illuminator that allows fluorescent time-lapse observation of dynamic events following photo activation or photo conversion.

With the integration of the laser TIRF illuminator and photo activation unit, both functions are now combined in one microscope. The user can switch between the two functions with ease.

Use of an optional back port enables multiple wavelength FRET imaging with multiple cameras. Moreover, by adding an eyepiece tube base unit with a side port, a maximum of five imaging ports* including left, right and bottom ports are available. (*With Ti-E/B model with bottom port)

The flagship model that is fully motorized for automated multimode image techniques and acquisition
(A model with a bottom port is also available.)

The universal model with the potential for diverse laser illuminators and motorized components
(A model with a bottom port is also available.)

The basic model that can be dedicated to specific tasks