Attend ISSCR

June 12-15, 2013 Boston, MA USA

The ISSCR's annual meeting has become the world's premier stem cell research event. The meeting serves as the largest forum for stem cell and regenerative medicine professionals from around the world. Nikon will present products and applications at the event. It will also offer the presentation Innovation Showcase.

The product will be on display. | Exhibition Booth Venue : LEVEL ZERO HALL A Booth No. 315

Innovation Showcase

Professor Lee L. Rubin, PhD

  • SPEAKER:
    Professor Lee L. Rubin, PhD

    Director of HSCI's Therapeutic Screening Center Professor in Harvard University, USA

  • DATE: Friday, June 14
  • TIME: 11:45 a.m. – 12:15 p.m.
  • VENUE: Room 258 A-C

Applications

Nikon BioStation CT realizes long term time-lapse imaging and advanced analysis not only on fluorescent images but also Phase contrast images.

Imaging of the direct induction from mouse fibroblasts to Neural stem cells and neurons

Stem Cells. 2012 Jun;30(6) : 1109-19

  • Courtesy
    Professor Hideyuki Okano and Dr. Takeshi Matsui Department of Physiology, Keio University School of Medicine
  • Magnification : 4X
  • Culture period : 18 days
  • Imaging interval : 4 hours

Neural differentiation analysis

Typical iPS colony formation movie

Imaging of reprogramming process

Human iPS colonies prediction within first 72 hours of colony formation

Features

Nikon BioStation CT is an advanced, automated cell culture observation instrument specialized for stem cell research.
Ideally suited for live cell imaging, BioStation CT maintains a stable environment during microscopic image acquisition.

Live Cell Analysis

Whole well scan imaging enables clear detection of an iPS colony, which is difficult to detect because of its low induction efficiency. Combination with advanced image analysis technology enable to be revolutionary new approach and lead to improvements in cell characterization methodologies and a better understanding of the reprogramming and differentiation process. Imaging and analysis become processed automatically by separate PC with no stress. The left Figure shows the result of automated classification into iPS and non-iPS cell colonies from the phase contrast images. (The entire area of a 100mm dish)

Multi Sample

BioStation CT can incubate total 30 vessels in case of well plate at once. Researchers can examine the differences in each culture vessels.

Vessel Type

User can use various culture vessels (Well-plate, dish, flask)

Stable Cultivation

Temperature, Humidity, CO2 concentration and O2 concentration are controlled by accurate sensor and feedback system.

User's List

  • Harvard Stem cell Institute
  • Morgridge Institute
  • Diabetes Center
  • UCR Stem Cell Core Facility
  • USC Stem Cell Core
  • Newcastle University
  • University Hospital Bern
  • Gurdon Institute
  • Masaryk University
  • China Agricultural University
  • Kyoto University
  • Keio University
  • National Institute of Biomedical
  • Innovation

Articles

Neural Stem Cells Directly Differentiated from Partially Reprogrammed Fibroblasts Rapidly Acquire Gliogenic Competency

Takeshi Matsui, Morito Takano, Kenji Yoshida, Soichiro Ono, Chikako Fujisaki, Yumi Matsuzaki, Yoshiaki Toyama, Masaya Nakamura, Hideyuki Okano, Wado Akamatsu
Stem Cells. 2012 Jun;30(6):1109-19

Protein Kinase C Regulates Human Pluripotent Stem Cell Self-Renewal

Masaki Kinehara, Suguru Kawamura, Daiki Tateyama, Mika Suga, Hiroko Matsumura, Sumiyo Mimura, Noriko Hirayama, Mitsuhi Hirata, Kozue Uchio-Yamada, Arihiro Kohara, Kana Yanagihara, Miho K. Furue
PLOS ONE. January 2013 | Volume 8 | Issue 1 | e54122

Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors

Kunihiko Hiramatsu, Satoru Sasagawa, Hidetatsu Outani, Kanako Nakagawa, Hideki Yoshikawa, and Noriyuki Tsumaki
J Clin Invest. 2011;121(2):640–657

Efficient and Reproducible Myogenic Differentiation from Human iPS Cells: Prospects for Modeling Miyoshi Myopathy In Vitro

Akihito Tanaka, Knut Woltjen, Katsuya Miyake, Akitsu Hotta, Makoto Ikeya, Takuya Yamamoto, Tokiko Nishino, Emi Shoji, Atsuko Sehara-Fujisawa, Yasuko Manabe, Nobuharu Fujii, Kazunori Hanaoka, Takumi Era, Satoshi Yamashita, Ken-ichi Isobe, En Kimura, Hidetoshi Sakurai
PLOS ONE. April 2013 | Volume 8 | Issue 4 | e61540

USA
Nikon Instruments Inc.

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Phone: +1-631-547-8500 : +1-800-52-NIKON (within the U.S.A. only)
Fax: +1-631-547-0306
E-mail: nikoninstruments@nikon.net

Europe
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Phone: +31-20-7099-000
Fax: +31-20-7099-298

ASIA
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Phone: +81-3-3216-2375
Fax: +81-3-3216-2385
E-mail: Support.Biostation@nikon.com

Nikon Instruments (Shanghai) Co., Ltd.

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Phone: +86-21-6841-2050
Fax: +86-21-6841-2060

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