Our major finding is the discovery of an early pattern predictive of iPS colony formation that occurs within the first 72 hours after a colony is first discernible.
Fully Reprogrammed iPS colonies form as the steady emergence of a smaller dense core (Blue area) than non-iPS colonies.
Analysis software CL-Quant's segmentation procedure and used for detection, and are insets of the Phase contrast images (green square).
The graph shows the detected counts and areas in the first 72 hours after each colony can be first observed.
This movie can be acquired by Nikon BioStation CT and reviewed in analysis software CL-Quant.
Professor Lee L. Rubin, PhD
Director of HSCI's Therapeutic Screening Center in Harvard University